800 MHz NMR Spectrometer
The 800 MHz NMR spectrometer is currently the highest field NMR spectrometer in the state of Oregon. The instrument is a 4-channel spectrometer equipped with a specially designed 5 mm TCI cryoprobe with Z-axis gradient, enabling improved sensitivity for 1H, 19F, 13C and 15N detected solution state experiments. In addition, the system incorporates a 24 well sample case for improved automation. This spectrometer will primarily be used for the analysis of biological samples, leading to advances in research areas such as Alzheimer’s Disease and Cancer. Instrument time on the 800 MHz spectrometer is allocated via request.
Cryoprobe Complete Specs
Location: LPSC 175
700 MHz NMR Spectrometer
This spectrometer features high sensitivity for natural products and chemistry. The instrument is a 2-channel spectrometer equipped with a 5 mm carbon observe cryoprobe with Z-axis gradient, providing high sensitivity for solution state carbon experiments. The spectrometer is primarily used for the analysis and characterization of natural products and newly synthesized organic compounds. Also available for this spectrometer are BBO and BBI probes. This instrument is one of the most sensitive carbon-detecting spectrometers in the world - capable of measuring a high quality carbon spectrum on 1000+ molecular weight samples containing as little as 0.1 mg.
Scheduling Guidelines: One hour maximum, Tuesday and Thursday, noon to 10 pm, one hour maximum Monday, Wednesday and Friday, noon to 7pm. Longer runs possible on weekends after consultation with NMR facility staff.
Location: LPSC 175
500 MHz NMR Spectrometer
The 500 MHz instrument is a 4-channel spectrometer used for a wide range of solution state applications. A 5 mm BBOF probe and 5mm TXI triple resonance (HCN) probe, both equipped with Z-axis gradients, are available for this spectrometer. The TXI and BBO probes are available according to the schedule found here.
Scheduling Guidelines (when BBO is installed): One hour maximum, Monday through Friday, noon to 6 pm.
Scheduling Guidelines (when TXI is installed): No Restrictions
Location: Gilbert Addition 101
400 MHz NMR Spectrometers (2)
These instruments are 2-channel NMR spectrometers used for solution state analysis. Available probes include 5mm BBO and 5 mm BBI probes, all with Z-axis gradients.
Robinson(LPSC): Monday through Friday, noon to 10 pm: No more than 2 consecutive 10 minute slots (20 minutes) per person per session.
Diels(ATAMI): No Restrictions
Location: LPSC 175 (Robinson), ATAMI (Diels)
The facility also operates a Beckman Optima XL-A analytical Ultracentrifuge. This instrument is used to characterize particle sizes and behavior in solution. It is particularly valuable for the quantitative analysis of biomolecules, such as proteins and nucleic acids. It has broad applications in biophysics, such as determining protein homogeneity, polydispersity, association constants and equilibrium thermodynamic properties. In many cases, analysis with analytical ultracentrifugation is a desirable first step to characterizing a new protein sample, prior to NMR based structural analysis. Please contact Dr. Patrick Reardon for further information about accessing this instrument