Using a combination of biophysical methods, structural analysis by NMR and electron microscopy, and cellular transcription assays, we developed a model that proposes a concerted role of intrinsic disorder and multiple LC8 binding events in regulating LC8 transcription.
The jizanpeptins are depsipeptides isolated from a Red Sea Symploca cyanobacterium possessing potent and specific trypsin inhibition. These 1100 Da compounds were isolated in amounts ranging from 300 to 1000 micrograms making structure elucidation a significant challenge.
This paper describes the biochemical and structural characterization of the proposed common intermediate found during the biosynthesis of triazine-containing natural products. This is a further example of how common intermediates can be diversified by microorganisms into different products.
Recent work from the Barbar Lab published in the journal Structure provides novel atomic-level insights into the effect of phosphorylation in two broad and intertwined scientific areas: regulation of the molecular motor dynein and intrinsically disordered proteins.