TitleThe LC8-RavP ensemble Structure Evinces A Role for LC8 in Regulating Lyssavirus Polymerase Functionality.
Publication TypeJournal Article
Year of Publication2019
AuthorsJespersen NE, Leyrat C, Gérard FC, Bourhis J-M, Blondel D, Jamin M, Barbar E
JournalJ Mol Biol
Volume431
Issue24
Pagination4959-4977
Date Published2019 Dec 06
ISSN1089-8638
Abstract

The rabies and Ebola viruses recruit the highly conserved host protein LC8 for their own reproductive success. In vivo knockouts of the LC8 recognition motif within the rabies virus phosphoprotein (RavP) result in completely nonlethal viral infections. In this work, we examine the molecular role LC8 plays in viral lethality. We show that RavP and LC8 colocalize in rabies infected cells, and that LC8 interactions are essential for efficient viral polymerase functionality. NMR, SAXS, and molecular modeling demonstrate that LC8 binding to a disordered linker adjacent to an endogenous dimerization domain results in restrictions in RavP domain orientations. The resulting ensemble structure of RavP-LC8 tetrameric complex is similar to that of a related virus phosphoprotein that does not bind LC8, suggesting that with RavP, LC8 binding acts as a switch to induce a more active conformation. The high conservation of the LC8 motif in Lyssavirus phosphoproteins and its presence in other analogous proteins such as the Ebola virus VP35 evinces a broader purpose for LC8 in regulating downstream phosphoprotein functions vital for viral replication.

DOI10.1016/j.jmb.2019.10.011
Alternate JournalJ. Mol. Biol.
PubMed ID31634467
PubMed Central IDPMC7060403
Grant ListR01 GM084276 / GM / NIGMS NIH HHS / United States
S10 OD018518 / OD / NIH HHS / United States