TitleExpanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4.
Publication TypeJournal Article
Year of Publication2020
AuthorsVideau P, Wells KN, Singh AJ, Eiting J, Proteau PJ, Philmus B
JournalACS Synth Biol
Volume9
Issue1
Pagination63-75
Date Published2020 Jan 17
ISSN2161-5063
Abstract

Cyanobacteria are prolific producers of natural products, and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters, and here we present the use of sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native 7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by coconjugation.

DOI10.1021/acssynbio.9b00334
Alternate JournalACS Synth Biol
PubMed ID31846576