TitleChemical, Bioactivity, and Biosynthetic Screening of Epiphytic Fungus .
Publication TypeJournal Article
Year of Publication2020
AuthorsGonzález-Montiel GA, Kaweesa EN, Feau N, Hamelin RC, Stone JK, Loesgen S
JournalMolecules
Volume25
Issue10
Date Published2020 May 19
ISSN1420-3049
KeywordsAnti-Bacterial Agents, Ascomycota, Biosynthetic Pathways, Cell Proliferation, Colonic Neoplasms, Genome, Fungal, Gram-Positive Bacteria, HCT116 Cells, Humans, Naphthoquinones, Secondary Metabolism
Abstract

We report the first secondary metabolite, 8,8'-bijuglone, obtained from pure cultures of the slow growing Douglas fir- ( var. ) foliage-associated fungus . The quinone was characterized using extensive LC/MS and NMR-based spectroscopic methods. 8,8'-Bijuglone exhibited moderate antibiotic activity against Gram-positive pathogens and weak cytotoxic activity in the NCI-60 cell line panel and in our in-house human colon carcinoma (HCT-116) cell line. An analysis of the fungal genome sequence to assess its metabolic potential was implemented using the bioinformatic tool antiSMASH. In total, 36 putative biosynthetic gene clusters were found with a majority encoding for polyketides (17), followed by non-ribosomal peptides (14), terpenes (2), ribosomal peptides (1), and compounds with mixed biosynthetic origin (2). This study demonstrates that foliage associated fungi of conifers produce antimicrobial metabolites and suggests this guild of fungi may present a rich source of novel molecules.

DOI10.3390/molecules25102358
Alternate JournalMolecules
PubMed ID32438585
PubMed Central IDPMC7287617
Grant ListCHE 1808717 / / National Science Foundation /