TitleAccessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions.
Publication TypeJournal Article
Year of Publication2022
AuthorsVesely CHoang, Reardon PN, Yu Z, Barbar E, Mehl RA, Cooley RB
JournalJ Biol Chem
Volume298
Issue12
Pagination102613
Date Published2022 Dec
ISSN1083-351X
KeywordsCOVID-19, Escherichia coli, Humans, Magnetic Resonance Spectroscopy, Phosphoserine, Recombinant Proteins, SARS-CoV-2, Serine
Abstract

Phosphoserine (pSer) sites are primarily located within disordered protein regions, making it difficult to experimentally ascertain their effects on protein structure and function. Therefore, the production of N- (and C)-labeled proteins with site-specifically encoded pSer for NMR studies is essential to uncover molecular mechanisms of protein regulation by phosphorylation. While genetic code expansion technologies for the translational installation of pSer in Escherichia coli are well established and offer a powerful strategy to produce site-specifically phosphorylated proteins, methodologies to adapt them to minimal or isotope-enriched media have not been described. This shortcoming exists because pSer genetic code expansion expression hosts require the genomic ΔserB mutation, which increases pSer bioavailability but also imposes serine auxotrophy, preventing growth in minimal media used for isotopic labeling of recombinant proteins. Here, by testing different media supplements, we restored normal BL21(DE3) ΔserB growth in labeling media but subsequently observed an increase of phosphatase activity and mis-incorporation not typically seen in standard rich media. After rounds of optimization and adaption of a high-density culture protocol, we were able to obtain ≥10 mg/L homogenously labeled, phosphorylated superfolder GFP. To demonstrate the utility of this method, we also produced the intrinsically disordered serine/arginine-rich region of the SARS-CoV-2 Nucleocapsid protein labeled with N and pSer at the key site S188 and observed the resulting peak shift due to phosphorylation by 2D and 3D heteronuclear single quantum correlation analyses. We propose this cost-effective methodology will pave the way for more routine access to pSer-enriched proteins for 2D and 3D NMR analyses.

DOI10.1016/j.jbc.2022.102613
Alternate JournalJ Biol Chem
PubMed ID36265582
PubMed Central IDPMC9678770
Grant ListRM1 GM144227 / GM / NIGMS NIH HHS / United States